Molecular characterization of a novel repetitive element from Pneumocystis carinii from rats
- 1 February 1993
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 31 (2) , 244-8
- https://doi.org/10.1128/jcm.31.2.244-248.1993
Abstract
A repetitive DNA sequence, Rp-alpha, was isolated from rat-derived Pneumocystis carinii. The genome of rat-derived P. carinii contained 10 to 15 copies of Rp-alpha, which were located on most chromosomes, but no Rp-alpha could be detected in P. carinii derived from either humans or mice. Sequence analysis of two copies of the repeat showed them to be related but distinct. Each of them contained several copies of the 9-base sequence TAACCCTAA, arranged as direct repeats. Oligonucleotides consisting of multimers of this 9mer hybridized to the same set of chromosomes recognized by cloned copies of the Rp-alpha repeat. When used in DNA fingerprinting, the Rp-alpha repeat was capable of distinguishing between different isolates of rat-derived P. carinii.Keywords
This publication has 22 references indexed in Scilit:
- Cloning and sequence of a β‐tubulin cDNA from Pneumocystis carinii: possible implications for drug therapyMolecular Microbiology, 1992
- Mitochondrial gene sequences show fungal homology for Pneumocystis cariniiMolecular Microbiology, 1991
- In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAsNature, 1990
- An electrophoretic karyotype and assignment of ribosomal genes to resolved chromosomes of Pneumocystis cariniiMolecular Microbiology, 1989
- Cloning of human telomeres by complementation in yeastNature, 1989
- In Vitro Studies ofPneumocystis cariniiThe Journal of Protozoology, 1989
- Phylogenetic association of Pneumocystis carinii with the ‘Rhizopoda/Myxomycota/Zygomycota group’ indicated by comparison of 5S ribosomal RNA sequencesMolecular and Biochemical Parasitology, 1989
- Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the FungiNature, 1988
- Pneumocystis carinii and Toxoplasma gondii Infections in Patients with AIDSClinical Infectious Diseases, 1986
- Identification of a telomeric DNA sequence in Trypanosoma bruceiCell, 1984