Immunophenotypic Analysis of Cells Isolated from Bone Marrow Biopsies in Patients with Failed Bone Marrow Aspiration ('dry tap')
- 1 April 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 93 (4) , 545-548
- https://doi.org/10.1093/ajcp/93.4.545
Abstract
A method for the immunophenotypic analysis of bone marrow cells in cases of failed bone marrow aspiration is described. Cell suspensions are obtained by mechanical disaggregation of bone marrow core biopsies. The isolated cells are stained with the appropriate antibodies and analyzed by flow cytometry. The usefulness of the method is illustrated by presenting immunophenotypic data obtained in eight consecutive cases accessioned by the authors' laboratory. The method is simple and reproducible. It allows for parallel morphologic examination with Romanovskitype stains and is capable of generating multivariate, quantitative, immunophenotypic data useful in the diagnosis of leukemia and lymphoma.This publication has 3 references indexed in Scilit:
- Immunophenotyping in the classification of acute leukemia in adults. Interpretation of multiple lineage reactivityCancer, 1989
- CONTRIBUTION OF IMMUNOPHENOTYPE TO THE CLASSIFICATION AND DIFFERENTIAL DIAGNOSIS OF ACUTE LEUKAEMIAThe Lancet, 1985
- THE IMMUNOLOGICAL PHENOTYPING OF BONE-MARROW BIOPSIES AND ASPIRATES - FROZEN SECTION TECHNIQUES1982