Molekulargewichtsbestimmungen durch polyacrylamidgelelektrophorese unter verwendung eines linearen gelgradienten

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Abstract
A new method for molecular weight determination using polyacrylamidegel electrophoresis in a linear gel‐concentration‐gradient (3–20°) is described. Plotting the log of molecular weights of several standard proteins against distance of migration or against 3 log of rate of n‐dgration reveal linear relationships in ranges of 50.000 – 200.000 or 100.000 – 400.000 Daltons respectively. On this basis, a simple method for molecular weight determination of proteins (accuracy ± 5°) has been devised. The method can also be applied for an individual protein in protein mixtures using specific staining procedures.