Evidence that poly(ADP-ribose) polymerase is involved in the loss of NAD from cultured rat liver cells
- 15 February 1982
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 202 (2) , 551-553
- https://doi.org/10.1042/bj2020551
Abstract
Rat hepatocytes cultured from 24h lose 60% of their NAD content. By using the differential response to inhibitors of the two major enzymes that catabolize NAD in mammalian cells, it is shown that poly(ADP-ribose) polymerase is responsible for the loss of NAD. The relevance of this observation to the use of cultured hepatocytes for the study of DNA repair induced by carcinogens is discussed.Keywords
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