Tn10-encoded tet repressor can regulate an operator-containing plant promoter.
- 1 March 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (5) , 1394-1397
- https://doi.org/10.1073/pnas.85.5.1394
Abstract
The Tn10-encoded tet repressor-operator system was used to regulate transcription from the cauliflower mosaic virus (CaMV) 35S promoter. Expression was monitored in a transient assay system by using electric field-mediated gene transfer ("electroporation") into tobacco protoplasts. The tet repressor, being expressed in the plant cells under the control of eukaryotic transcription signals, blocks transcription of a CaMV 35S promoter chloramphenicol acetyltransferase (cat) fusion gene when the two tet operators flank the "TATA" box. In the presence of the inducer tetracycline, expression is restored to full activity. Location of the operators 21 base pairs downstream of the transcription start site does not significantly affect transcription in the presence of the repressor. These experiments show that a prokaryotic regulatory protein can function in plant cells. The tet repressor-operator complex may be useful for specifically inducing transferred genes at different stages of plant development.This publication has 26 references indexed in Scilit:
- Gene regulation by proteins acting nearby and at a distanceNature, 1986
- Organ-Specific and Light-Induced Expression of Plant GenesScience, 1986
- Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by fluorescence measurementsJournal of Molecular Biology, 1986
- Transcriptional enhancers play a major role in gene expressionBioEssays, 1986
- 5′ end of hmg CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcriptionCell, 1985
- Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoterNature, 1985
- A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast geneNature, 1984
- Nucleotide sequence of the gene, protein purification and characterization of the pSC101-encoded tetracycline resistance-gene-repressorGene, 1984
- Control of expression of the Tn10-encoded tetracycline resistance operon II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory regionJournal of Molecular Biology, 1984
- Overlapping divergent promoters control expression of Tn10 tetracycline resistanceGene, 1983