The organic nitrogen exigency of and effects of manganese on coremia production in Penicillium clavigerum and Penicillium claviforme

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Abstract
The production of mycelium and conidia of Penicillium clavigerum and Penicillium claviforme is severely restricted if an amino acid is not present in a maltose–mineral salts medium. This requirement can be satisfied by any L-amino acid, although the optimum quantity varies with the type of amino acid. When ammonium–nitrogen is substituted for nitrate–nitrogen or amino acid–nitrogen in the P. clavigerum medium, the formation of coremia and conidia is prevented, mycelial growth is inhibited, and an orange intracellular pigment is produced. The numbers of coremia and conidia produced by both organisms are regulated by the quantity of manganese in the media.

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