Mutational Analysis of Ligand Recognition by Tcp, the Citrate Chemoreceptor of Salmonella enterica Serovar Typhimurium
- 1 March 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (5) , 1437-1441
- https://doi.org/10.1128/jb.182.5.1437-1441.2000
Abstract
The chemoreceptor Tcp mediates taxis to citrate. To identify citrate-binding residues, we substituted cysteine for seven basic or polar residues that are chosen based on the comparison of Tcp with the well-characterized chemoreceptors. The results suggest that Arg-63, Arg-68, Arg-72, Lys-75, and Tyr-150 (and probably other unidentified residues) are involved in the recognition of citrate.Keywords
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