Co-operative binding of oxytocin to bovine neurophysin II
- 1 May 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 147 (2) , 377-379
- https://doi.org/10.1042/bj1470377
Abstract
The interaction of oxytocin with bovine neurophysin II in 0.1 M-sodium phosphate, pH 5.8, was investigated by equilibrium-dialysis and sedimentation studies. Sigmoidality of the binding curve is attributed to isomerization, either hormone-induced or pre-existing, with preferential binding of oxytocin to one isomeric state. Results are consistent with a binding equation of the form r = (2P[S]+2PQ[S]2)/(1+2P[S]+PQ[S]2) and values of 0.7 X 10(5)M-1 and 1.3 X 10(5)M-1 for P and Q respectively. The significance of these two parameters in relation to current theories of allostery is also discussed.Keywords
This publication has 17 references indexed in Scilit:
- Synthesis of oxytocin: Suppression of dimer formationCellular and Molecular Life Sciences, 1973
- Physical and Chemical Properties of the Bovine NeurophysinsJournal of Biological Chemistry, 1971
- Allosteric interactions in aspartate transcarbamylase. I. Binding of specific ligands to the native enzyme and its isolated subunitsBiochemistry, 1968
- The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and -II as complexes with [8-arginine]-vasopressinBiochemical Journal, 1968
- The binding of oxytocin and oxytocin analogues by purified bovine neurophysins.Proceedings of the National Academy of Sciences, 1966
- Comparison of Experimental Binding Data and Theoretical Models in Proteins Containing Subunits*Biochemistry, 1966
- On the nature of allosteric transitions: A plausible modelJournal of Molecular Biology, 1965
- The interaction of ribonuclease with purine and pyrimidine phosphates I. Binding of adenosine 5′-monophosphate to ribonucleaseBiochimica et Biophysica Acta, 1962
- L'ocytocine et la vasopressine du mouton: Reconstitution d'un complexe hormonal actifBiochimica et Biophysica Acta, 1959
- Purification of Oxytocin and Vasopressin by Way of a Protein ComplexJournal of Biological Chemistry, 1958