THE ESTRIN CONTENT OF THE FOLLICULAR FLUID AND URINE OF THE MARE AND ITS RELATION TO PHENOMENA OF THE ESTRUAL CYCLE1

Publisher Website
Google Scholar
Abstract
The ovaries of 11 mares were palpated per rectum at different stages of the estrous cycle, then desirable follicles were punctured and contents collected for subsequent chemical analyses and chemical assay of the estrogen conc. The vol. of the fluid obtained ranged from 1 cc.-112 cc, varying with the age, diam. of follicles punctured and phase of cycle in which they were punctured. With one exception the greatest quantity of fluid was obtained during estrus. The protein content of the fluid averaged 0.57% and was approx. the same for all samples analysed. The sp. gr. varied between 1.008 and 1.025, but in most cases was nearer the lower figure. The significance of these results was discussed. The total estrogens in the fluid recovered from the individual follicles was 1/3 as great in the non-estrus phases of the cycle as during estrus. However, the estrogen conc. per unit vol. of fluid was constant throughout the cycle. The importance of the latter observation was discussed. A total of 128 urine samples collected from 24 mares at 2 to 3 days intervals during the entire cycle were assayed by a chemical method for their total estrogen content. Evidence indicating 2 peaks in estrogen excretion in the non-pregnant mare[long dash]one during estrus and one between the 10th and 15th day[long dash]was presented. Citation was made of a marked rise in estrogen excretion by the pregnant mare beginning as early as 30 days after conception occurred.

This publication has 2 references indexed in Scilit: