LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney
- 1 August 1988
- journal article
- research article
- Published by Springer Nature in Journal of Molecular Histology
- Vol. 20 (8) , 427-432
- https://doi.org/10.1007/bf01002428
Abstract
Summary This work describes a method for the immunolocalization of laminin on 1μm-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50°C whereas LR-Gold was polymerized at −25°C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.Keywords
This publication has 8 references indexed in Scilit:
- Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-GoldHistochemistry and Cell Biology, 1988
- Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidneyHistochemistry and Cell Biology, 1987
- Immunocytochemical demonstration of serine:pyruvate aminotransferase in peroxisomes and mitochondria of rat kidneyHistochemistry and Cell Biology, 1985
- Immunocytochemical Enhancement of Basement Membrane Antigens by Pepsin: Applications in Diagnostic PathologyAmerican Journal of Clinical Pathology, 1984
- A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscopeJournal of Molecular Histology, 1983
- Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissuesHistochemistry and Cell Biology, 1982
- POST-EMBEDDING IMMUNOLABELING - SOME EFFECTS OF TISSUE-PREPARATION ON THE ANTIGENICITY OF PLANT-PROTEINS1982
- Laminin–a glycoprotein from basement membranes.Journal of Biological Chemistry, 1979