A Review of methods for the Determination of Hold-up Volume in Modern Liquid Chromatography

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Abstract
Arguably, the most important parameter in modern liquid chromatography is the hold-up (or dead) volume, VM, the volume of mobile phase contained within the chromatographic system between the sample injector and the detector. Without this knowledge many dependent parameters such as capacity factor (k), selectivity (α), and resolution (Rg) cannot be computed (104, 15, 78). These data are of the utmost importance for the optimization of conditions for the separation of complex mixtures and the identification of solute bands.