Deoxyribonucleic acid sequence of a gene from the Pseudomonas transposon TN501 encoding mercuric reductase

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Abstract
The nucleotide sequence of the merA gene from the Hg-resistance transposon Tn501 and have the structure of the gene product, mercuric reductase, was predicted. The DNA sequence predicts a polypeptide of MW 58,660, the primary structure of which shows strong homologies to glutathione reductase and lipoamide dehydrogenase, but mercuric reductase contains as additional N-terminal region that may form a separate domain. The implications of these comparisons for the tertiary structure and mechanism of mercuric reductase are discussed. The DNA sequence presented here has an overall G + C content of 65.1 mol%, typical of the bulk DNA of P. aeruginosa from which Tn501 was originally isolated. Analysis of the codon usage in the merA gene shows that codons with C or G at the third position are preferentially utilized.

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