Selective Extraction and Quantitation of Polyoxyethylene Detergents and its Application in Protein Determination

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Abstract
A simple, rapid and selective method for the nearly quantitative extraction of high amounts (10% w/v) of polyoxyethylene nonionic detergents like Triton X-100R is presented based on solvent extraction with 1, 2-dichloroethane. Protein determination after extraction of protein containing solutions can be accomplished using the Bradford assay1 or other standard methods. High protein recoveries can be obtained. The detergent analysis by HPLC using reversed-phases is not disturbed in the presence of proteins and therefore provides a fast and elegant method for the exact quantitation of nonionic detergents.