Cryopreservation of flagellar mutants of Chlamydomonas reinhardtii

Abstract
Ten strains of C. reinhardtii flagellar mutants were successfully cryopreserved using 2 cryogenic freezing methods. Dimethylsulfoxide (DMSO) was found to be the only effective cryoprotectant of those tested. After recovery, cells were viable and morphologically indistinguishable from non-frozen cells. No recovery was obtained after lyophilization.

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