Cooperative Binding of Porphyrin by Anti-Porphyrin Antibodies

Abstract
One of the monoclonal antibodies specific for MESO-tetrakis(4-carboxyphenyl)porphine (TCPP) causes a large shift of the Soret band of TCPP to a longer wavelength and large induced Cotton effects (ICD) on TCPP. Quantitative analyses of the binding of TCPP to the antibody by ICD show that not only one-to-one binding but two-to-one (binding site: TCPP) binding occurs in excess of the antibody over TCPP.

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