Glycopeptide-Resistant Enterococcus faecium BM4416 Is a VanD-Type Strain with an Impaired d -Alanine: d -Alanine Ligase

Abstract
VanD-type Enterococcus faecium BM4416 was constitutively resistant to vancomycin and to teicoplanin by synthesis of peptidoglycan precursors ending in d -alanyl– d -lactate. Like E. faecium BM4339, the only VanD-type strain described so far, BM4416 produced an impaired d -alanine: d -alanine ligase. Unlike for BM4339, which had a 5-bp insertion in ddl , inactivation of the gene in BM4416 was due to insertion of IS 19 .