Prostaglandin H2‐derived adducts of proteins correlate with Alzheimer's disease severity
Open Access
- 30 June 2005
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 94 (4) , 1140-1145
- https://doi.org/10.1111/j.1471-4159.2005.03264.x
Abstract
The formation of cyclooxygenase‐derived lipid adducts of protein in brains of patients who had Alzheimer's disease has been investigated. The enzymatic product of the cyclooxygenases, prostaglandin H2, rearranges in part to highly reactive γ‐ketoaldehydes, levuglandin (LG) E2 and LGD2. These γ‐ketoaldehydes react with free amines on proteins to yield a covalent adduct. Utilizing analysis of the levuglandinyl‐lysine adducts by liquid chromatography‐tandem mass spectrometry, we now find that this post‐translational modification is increased significantly in the hippocampus in Alzheimer's disease. The magnitude of the increase correlates with the pathological evidence of severity.Keywords
This publication has 31 references indexed in Scilit:
- Synergistic and antagonistic roles of the Sonic hedgehog N- and C-terminal lipidsDevelopment, 2004
- Levuglandinyl Adducts of Proteins Are Formed via a Prostaglandin H2 Synthase-dependent Pathway after Platelet ActivationJournal of Biological Chemistry, 2003
- Prostaglandin H2 (PGH2) accelerates formation of amyloid β1−42 oligomersJournal of Neurochemistry, 2002
- Effects of reactive γ‐ketoaldehydes formed by the isoprostane pathway (isoketals) and cyclooxygenase pathway (levuglandins) on proteasome functionThe FASEB Journal, 2002
- A subset of NSAIDs lower amyloidogenic Aβ42 independently of cyclooxygenase activityNature, 2001
- Formation of Highly Reactive γ-Ketoaldehydes (Neuroketals) as Products of the Neuroprostane PathwayJournal of Biological Chemistry, 2001
- Regional distribution of cyclooxygenase-2 in the hippocampal formation in Alzheimer's diseaseJournal of Neuroscience Research, 1999
- Reactions of 4-Hydroxy-2(E)-nonenal and Related Aldehydes with Proteins Studied by Carbon-13 Nuclear Magnetic Resonance SpectroscopyChemical Research in Toxicology, 1998
- Generation of pyrroles in the reaction of Levuglandin E2 with proteinsThe Journal of Organic Chemistry, 1994
- Prostaglandin endoperoxides. 14. Solvent-induced fragmentation of prostaglandin endoperoxides. New aldehyde products from PGH2 and a novel intramolecular 1,2-hydride shift during endoperoxide fragmentation in aqueous solutionJournal of the American Chemical Society, 1984