DIFFERENCES IN LEUCYL-TRANSFER RNA'S AND SYNTHETASE IN SOYBEAN SEEDLINGS
- 1 January 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 62 (1) , 202-209
- https://doi.org/10.1073/pnas.62.1.202
Abstract
Charged leucyl-transfer RNA (leucyl-tRNA) of soybean cotyledons can be fractionated into six radioactive peaks on a Freon column. Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (species 5 and 6). Further, leucyl-tRNA(5 and 6) of the hypocotyl are present in small amounts in comparison to the cotyledon. Therefore, the rate-limiting quantities of synthetase and tRNA(5 and 6) obtained from the hypocotyl prevent the detection of these two species of tRNA when a homologous hypocotyl system is used. To measure the relative amounts of hypocotyl tRNA's, the cotyledon synthetase preparation is employed. Preliminary results show that 6-benzyladenine increases the acylation of leucyl-tRNA(5 and 6) and decreases acylation of tRNA(1) in the hypocotyl. Synthetase preparations from cytokinin-treated plants are not significantly affected.Keywords
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