THE SOLUBLE AND CYTOCHROME-LACKING NITRATE-REDUCING SYSTEM IN GERMINATING COTYLEDONS OF BEAN SEED EMBRYOS, VIGNA SESQUIPEDALIS*

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Abstract
A soluble nitrate reductase system was prepared by differential centrifugation at 2000, 20,000 and 107,000 X g, successively. The preparations contained nitrate reductase, capable of electron transferring from reduced phenosafranine to nitrate, flavine compound as an electron carrier, and formic dehydrogenase. In the presence of diphosphopyridine nucleotide the preparation oxidized formate anaerobically and reduced nitrate to nitrite. Cytochrome b was not detectable in the solution preparation, but it was distributed mainly in the precipitated fractions.

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