Influence of Age on Chromatin Transcription in Murine Tissues using an Heterologous and an Homologous RNA Polymerase
- 1 January 1976
- journal article
- research article
- Published by S. Karger AG in Gerontology
- Vol. 22 (3) , 111-123
- https://doi.org/10.1159/000212128
Abstract
No gross changes in the compositions of chromatin prepared from liver, kidney and brain were noted with age. A decreased age-dependent in vitro chromatin template activity was observed using an Escherichia coli RNA polymerase. This was paralleled by a reduction in the number of initiation sites on the chromatin for the enzyme. However, using a homologous RNA polymerase definite age-related specificities in template activity were found. When chromatin and enzyme were ‘age-matched’, maximal activity was observed. This level of activity was comparable in both 6 and 31-month-old mice, which suggested no impairment of function with age.Keywords
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