INVESTIGATIONS ON THE FLUOROMETRIC DETERMINATION OF MALIC AND SUCCINIC ACIDS IN APPLE TISSUE
- 1 October 1948
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 23 (4) , 443-454
- https://doi.org/10.1104/pp.23.4.443
Abstract
A photometric method was developed for the quantitative detn. of succinic and malic acids in mixed solns. and in certain plant tissues. Succinic and malic acids when heated with resorcinol and N2SO4 yield compounds which show intense fluorescence under specific conditions. Fluorometric separation of the succinic and malic acid derivs. was accomplished by controlling the exciting light and the reaction of the test soln. Dilute solns. of the succinic acid derivs. show an intense green fluorescence in acid reaction when illuminated by light of wave length 420-520 millimicrons. The malic acid deriv. shows virtually no fluorescence under these conditions but fluoresces a brilliant blue in basic soln. under u.-v. light of 320-420 millimicrons.This publication has 3 references indexed in Scilit:
- APPLICATION OF THE LIQUID EXTRACTION METHOD FOR THE DETERMINATION OF TOTAL ORGANIC ACIDS IN PLANT SAPPlant Physiology, 1942
- Determination of Succinic Acid in Plant TissuesIndustrial & Engineering Chemistry Analytical Edition, 1941
- Organic Acids in Plant TissuesIndustrial & Engineering Chemistry Analytical Edition, 1941