Distribution of sodium and potassium within cattle lens
- 1 May 1959
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 72 (1) , 126-133
- https://doi.org/10.1042/bj0720126
Abstract
The Na, K and water contents of the capsule plus adhering tissue, and of concentric layers of calf and cattle lens, were measured under various conditions. The average concentration of Na in calf or cattle lens was 15-21 m-molal, and of K 119-122 m-molal. The Na concentration in calf or cattle lens increased from about 12 m-molal in the nucleus to about 80 m-molal in the capsule plus adhering tissue. The K concentration was maximal in the cortical fibers just below the capsule. The excess of concentration of Na in the outermost zone was reduced by blotting the lens, and was abolished by washing the lens in water or 0.25 [image] sucrose. Storage of the excised lens at 4[degree] caused the Na to become more evenly distributed throughout the tissue water. Storage of the lens within the excised eye at 18[degree] for periods up to 18 hr. caused an inward penetration of Na from the aqueous humor, and a loss of K from the lens. The penetration started after storage for about 3 hr.Keywords
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