COMPLEMENT-FIXING ACTIVITY OF NORMAL RABBIT SERUM WITH RABBIT SPERMATOZOA AND SEMINAL PLASMA
Open Access
- 1 August 1960
- journal article
- Published by Bioscientifica in Reproduction
- Vol. 1 (3) , 268-282
- https://doi.org/10.1530/jrf.0.0010268
Abstract
Normal serum from male and female rabbits aged 1 o weeks or more possessed complement-fixing activity when mixed with rabbit semen, washed or epididymal spermatozoa, and the non-dialysable fraction of seminal plasma obtained from semen, but not with seminal plasma obtained from a vasectomized buck. Sera from younger animals did not have this activity. The active fraction in seminal plasma originated in the spermatozoa. Absorption with epididymal spermatozoa reduced the activity in two sera considerably, and slightly in a third. Other tests, i.e. passive cutaneous anaphylaxis (PCA) in the guinea-pig, precipitation tests, and agglutination or immobilization of spermatozoa in vitro, did not indicate the presence of antibody-like activity in normal sera.Keywords
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