A RAPID METHOD FOR THE LARGE SCALE PREPARATION OF CALF THYMUS DEOXYRIBONUCLEATE
- 1 November 1958
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 36 (11) , 1115-1119
- https://doi.org/10.1139/o58-120
Abstract
Isolation of deoxyribonucleate from a concentrated solution of thymonucleoprotein has been achieved by disruption of the protein nucleate bond with potassium thiocyanate, adsorption of the protein on celite, and alcohol precipitation of the deoxyribonucleate from aqueous salt solution. Highly polymerized nucleate can be obtained readily from 1 lb of calf thymus tissue in high yield.Keywords
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