Cytotoxicity of root canal sealers: a study using HeLa cells and fibroblasts
- 1 April 1984
- journal article
- Published by Wiley in International Endodontic Journal
- Vol. 17 (2) , 60-66
- https://doi.org/10.1111/j.1365-2591.1984.tb00382.x
Abstract
Summary. The relative cytotoxicity of five root canal sealers was measured by adding various concentrations of freshly mixed and set sealers to cultures of either HeLa or human fibroblast cells. The toxicity was determined by cell survival, which was measured by subsequent incorporation of [3H]‐thymidine into the nucleic acid components of each cell type used. The results showed that all sealers are cytotoxic in both fresh and set states. The ranking of toxicity of sealers on HeLa cells was similar to the one obtained for fibroblasts. Comparison of the results of this study with those of previous investigations questions the value of ranking sealers based only upon their effects in vitro.Keywords
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