NEMATOCIDAL ACTIVITY TO PRATYLENCHUS PENETRANS BY CULTURE FLUIDS FROM ACTINOMYCETES AND BACTERIA

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Abstract
The fluids from cultures of four isolates of Streptomyces sp. and two isolates of Bacillus sp. which were obtained from soil were found to be nematocidal (in vitro) to adults and larvae of Pratylenchus penetrans. Fluids from bacterial cultures were more nematocidal than those from actinomycete cultures, and the degree of activity was dependent upon both temperature and duration of incubation. Approximately 60% mortality resulted with bacterial fluids produced at 10 and 15 C, whereas those produced at 25 C caused 30% mortality. Approximately 40% mortality resulted with fluids produced by actinomycetes at 5, 10, or 15 C, and 15% mortality with those produced at 25 C. Activity was generally independent of the amount of cellular material produced, as greatest growth of the cultures occurred at 25 C.