Amino Acid Composition of Cockroach Hypertrehalosaemic Hormones
Open Access
- 1 February 1985
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 40 (1-2) , 42-46
- https://doi.org/10.1515/znc-1985-1-210
Abstract
Hypertrehalosaemic hormones I and II from the corpus cardiacum of the American cockroach (Periplaneta americana) were separated by reversed-phase high-performance liquid chromatography using a Nucleosil C18 column with a trifluoroacetic acid/acetonitrile gradient. The eluent was monitored at 206 nm and the hypertrehalosaemic activity detected by bioassay. The amino acid compositions of hypertrehalosaemic hormone I and II were determined after acid hydrolysis with HCl or methanesulfonic acid. Both neurohormones are octapeptides. Hypertrehalosaemic hormone I contained the amino acids Asp(2), Ser, Glu, Pro, Val, Phe and Trp, whereas hypertrehalosaemic hormone II contained the amino acid residues Asp, Thr(2), Glu, Pro, Leu, Phe and Trp.Keywords
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