LOCATING SALMONELLA RESISTANCE GENE ON MOUSE CHROMOSOME-1

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 37  (1) , 1-6
Abstract
The inherited resistance of inbred mouse strains to S. typhimurium injected s.c. is controlled primarily by a single gene designated Ity. Resistant mice have the dominant allele Ityr and sensitive mice are homozygous for the recessive allele Itys. The gene for resistance was located using readily distinguishable phenotypes as chromosome markers. Appropriate F1 backcross and F2 generations of hybrids between resistant and susceptible inbred strains of mice with or without the particular phenotypic markers, were tested both for susceptibility to infection and presence of the marker. Independent segregation of the characteristics eliminated all chromosomes except chromosome I. C57L mice resistant to S. typhimurium, Ityr Ityr and leaden, ln ln, located on chromosome 1, were crossed with BALB/c mice sensitive to S. typhimurium and non-leaden. In the F2 generation mice, ln always segregated with Ityr. Only 7 of 60 mice were sensitive to S. typhimurium, suggesting that Ityr is closely inbred to ln on chromosome 1. This will be of use in experiments with hybrid populations as predetermination of resistance or sensitivity to S. typhimurium without actually infecting the mice thus allowing experiments on the nature of the inheritance in unsensitized mice.