EFFECT OF INFLAMMATORY SERUM ON GLUCOSAMINE-C-14 INCORPORATION INTO BONE-MARROW GRANULOCYTES INVITRO

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 7  (7) , 389-396
Abstract
As a preliminary approach to developing a biochemical assay for detecting humoral regulators of [guinea pig] granulocyte maturation in the normal and inflammatory states, studies were carried out on the effects of normal and inflammatory sera on the incorporation of 14C-glucosamine into the glycoproteins of bone marrow granulocytes in vitro. Relative to normal serum, inflammatory serum had a marked stimulatory effect on 14C-glucosamine incorporation into these glycoproteins. This property of inflammatory serum reached a maximum at about 8 h after the initiation of inflammation in vivo and preceded the maximum increase in the mitotic activity of granulocyte precursors in the marrow by 18 h. Normal serum contained dialyzable and heat-sensitive nondialyzable factors that inhibited 14C-glucosamine incorporation into bone marrow granulocytes in vitro. The stimulatory effect of inflammatory serum is most likely due to a nondialyzable factor which is capable of blocking the effect of the inhibitors present in normal serum.