High Yields of DNA-Transfer into Mouse L-Cells by Electropermeabilization
Open Access
- 1 October 1985
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 40 (11-12) , 929-932
- https://doi.org/10.1515/znc-1985-11-1233
Abstract
DNA transfection in mouse L-cells was performed by means of the electropermeabilization technique (U. Zimmermann, G. Pilwat, and F. Riemann, Z. Naturforsch. 29, 304 (1974)). The plasmid pSV 2-neo used leads to neomycin-resistance in stably transfected L-cells. Optimized conditions resulted in high yields of clones at relatively low DNA concentration. The influence of temperature during pulse application and during the subsequent resealing process as well as the field parameters and medium composition are discussed.Keywords
This publication has 7 references indexed in Scilit:
- Gene transfer in lymphoid cells: expression of the Thy-1.2 antigen by Thy-1.1 BW5147 lymphoma cells transfected with unfractionated cellular DNA.Proceedings of the National Academy of Sciences, 1984
- Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.Proceedings of the National Academy of Sciences, 1984
- Gene transfer into mouse lyoma cells by electroporation in high electric fields.The EMBO Journal, 1982
- PENETRATION AND ENTRAPMENT OF LARGE PARTICLES IN ERYTHROCYTES BY ELECTRICAL BREAKDOWN TECHNIQUES1978
- Preparation of uniform haemoglobin free human erythrocyte ghosts in isotonic solutionBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Organspezifische Applikation von pharmazeutisch aktiven Substanzen über zellulare Trägersysteme / Organ Specific Application of Drugs by Means of Cellular Capsule SystemsZeitschrift für Naturforschung C, 1976
- Enzyme loading of electrically homogeneous human red blood cell ghosts prepared by dielectric breakdownBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976