A new type of plasmid from a wild isolate ofDictyosteliumspecies: the existence of closely situated long inverted repeats
Open Access
- 11 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (3) , 1097-1107
- https://doi.org/10.1093/nar/15.3.1097
Abstract
A circular plasmid having high copy number was found in a wild isolate of Dictyostelium species. Gel electrophoresis, electron microscopy and Southern blot hybridization revealed that the plasmid, named pDG1, is 4.5Kb(1.5µm) in size with closely situated long inverted repeats. The plasmid seems to be located in the nuclei. It was not a derivative of ribosomal DNA. The possible correlation of the plasmid with the putative intermediate DNA of retro-transposon DIRS-1 found in Dictyostelium discoideum is discussed.Keywords
This publication has 17 references indexed in Scilit:
- Spatial and temporal regulation of a foreign gene by a prestalk-specific promoter in transformed Dictyostelium discoideum.Molecular and Cellular Biology, 1986
- Sequence of Dictyostelium DIRS-1: An apparent retrotransposon with inverted terminal repeats and an internal circle junction sequenceCell, 1985
- Presence of nuclear associated plasmids in the lower eukaryote Dictyostelium discoideumJournal of Molecular Biology, 1985
- Mitochondrial class II introns encode proteins related to the reverse transcriptases of retrovirusesNature, 1985
- High-copy-number transformants and co-transformation in DictyosteliumGene, 1985
- Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans.Proceedings of the National Academy of Sciences, 1984
- Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cellsNature, 1981
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Structural organization of the genome of Dictyostelium discoideum: Analysis by EcoR1 restriction endonucleaseJournal of Molecular Biology, 1976
- A small nuclear precursor of messenger RNA in the cellular slime mold Dictyostelium discoideumJournal of Molecular Biology, 1973