Isolation of Amylase Inhibitor-producing Microorganism

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Abstract
An amylase inhibitor-producing microorganism was identified as a subspecies of Strepto- myces diastaticus from morphological and physiological studies and was named Streptomyces diastaticus subsp. amylostaticus No. 2476. When this strain was aerobically cultured in a shaking flask containing 100 ml of medium consisting of 4% corn starch, 2% soy bean flake extract, 0.3 % NaCl, 0.1 % K2HPO4, 0.05% MgSO4·7H2O, 0.001% FeS04 · 7H2O, 0.0001% CuSO4-5H2O, 0.0001% ZnSO4·7H2O, and 0.0001% MnS04 nH2O (pH 7.0) at 30°C, the highest inhibitory activity was obtained after 70 ~ 80 hr of cultivation. This amylase inhibitor (S-AI) had inhibitory activity on α-amylases and glucoamylase, but not on β-amylases and pullulanase.