In vitro Translation of the Bipartite Genomic RNA of Pea Early Browning Virus

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Abstract
Translation of the two genomic RNAs of a British strain (SP5) of pea early browning virus (PEBV-B) in rabbit reticulocyte lysates produced three principal polypeptides of Mr 165 .times. 103 (165K), 134K and 23K. The two largest polypeptides were produced by RNA-1 alone, and evidence is presented which suggests that the 165K polypeptide arises from the 134K product by a readthrough event. RNA-2 directed the translation of the 23K polypeptide, which was immunoprecipitated by anti-PEBV-B serum. A polypeptide of Mr 24.3K, produced by translation of the genomic RNAs of the closely related broad bean yellow band virus, was also immunoprecipitated by anti-PEBV-B serum, confirming the serological relationship between the two viruses.