MONOCLONAL-ANTIBODIES REACTING WITH ISOTYPIC AND OR ALLOTYPIC DETERMINANTS OF HUMAN-IGG

  • 1 January 1983
    • journal article
    • research article
    • Vol. 61  (2) , 126-132
Abstract
Mice were immunized with purified human IgG myeloma proteins and hybridomas were prepared using their spleen cells. Of the hybridomas, 1817 secreted anti-Ig antibodies. Several of them detected subclass-associated determinants. Eight different specificities were distinguished. The number of hybridomas in each category were the following: 3 anti-IgG1 (1.8% of all anti-Ig clones), 5 anti-IgG2 (0.3% of all anti-Ig clones), 2 anti-IgG3 (2.5% of all anti-Ig clones), 3 anti-IgG4 (12% of all anti-Ig clones), 12 anti-IgG1, IgG2, IgG3, 70 anti-IgG1, IgG2, IgG4, 2 anti-IgG2, IgG4 and, 7 anti-IgG2, IgG4 (1 out of 5 myelomas).

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