Prostaglandin F2α Binding to Bovine Ocular and Synthetic Melanins in Vitro
- 1 August 1989
- journal article
- research article
- Published by Wiley in Basic & Clinical Pharmacology & Toxicology
- Vol. 65 (2) , 100-103
- https://doi.org/10.1111/j.1600-0773.1989.tb01136.x
Abstract
The binding of 3H-prostaglandin F2α to bovine iris and synthetic melanin was studied in vitro using a ligand binding assay. Prostaglandin F2α was reversibly bound to both types of melanin. The binding was saturable and the Scatchard analysis revealed the existence of at least two binding sites with the corresponding KD values of 3.71 nM and 1.99 μM for natural and 4.99 nM and 0.19 μM for synthetic melanin, respectively. The high affinity Bmax values were 3.4 nM/g for natural and 2.5 nM/g for synthetic melanin. The dissociation of prostaglandin F2α from melanin after dilution of the complex was rapid and uniform.This publication has 10 references indexed in Scilit:
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