A new method of quantification of pipemidic acid by capillary zone electrophoresis with end-column amperometric detection

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Abstract
Capillary zone electrophoresis was employed for the determination of pipemidic acid using an end‐column amperometric detection with a carbon fiber microdisk array electrode, at a constant potential of −1.10 V vs. saturated calomel electrode. The optimum conditions of separation and detection were 1.2 × 10−4 mol/LNaOAc — 8.8 × 10−4 mol/LHOAc for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time. The limit of detection was 1.05 × 10−6 mol/L or 189 amol (S/N=3). The relative standard deviation was 0.31% for the migration time and 2.0% for the electrophoretic peak current. The method was applied to determining pipemidic acid in human serum.

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