Heteroduplex formation by recA protein: polarity of strand exchanges.
- 1 October 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (10) , 6149-6153
- https://doi.org/10.1073/pnas.78.10.6149
Abstract
Purified [Escherichia coli] recA protein promotes strand exchanges between linear duplex DNA and homologous circular single-stranded phage .vphi.X174 DNA that carries a short hybridized fragment. The mechanism of this strand exchange reaction was investigated. recA protein initiated strand exchanges by pairing the free end of the duplex fragment with the single-stranded DNA. Strand exchanges were polar and stable heteroduplex molecules were formed by the directional transfer of the (-) strands starting at 3'' termini.This publication has 23 references indexed in Scilit:
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