DNA binds neutrophil elastase and mucus proteinase inhibitor and impairs their functional activity
- 7 March 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 361 (2-3) , 265-268
- https://doi.org/10.1016/0014-5793(95)00173-7
Abstract
DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. DNA is a potent hyperbolic inhibitor of elastase. The polynucleotide-enzyme complex is partially active on synthetic substrates and on elastin. DNA strongly increases k diss and K i for the inhibition of elastase by mucus proteinase inhibitor. The above effects are all salt-dependent. At physiological ionic strength, DNA is a potent inhibitor of the elastolytic activity of elastase and increases k diss and K i for the elastase-mucus proteinase inhibitor interaction 160-fold and 100-fold, respectively.Keywords
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