Enhanced Lysosomal Enzyme Activity in Mouse Cells Treated with Bacterial Endotoxin in Vitro

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Abstract
Summary The specific activity of β-glu-curonidase in the granular fraction from whole spleen, adherent spleen cells, or peritoneal exudate cells of ICR mice was greater for samples from cell cultures treated with 10μg of endotoxin/ml in vitro than that for the corresponding untreated cultures. The endotoxin-stimulated elevation in β-glucu-ronidase and acid phosphatase activities was obtained with adherent spleen cells incubated in modified Eagle's medium and Hanks' balanced salt solution, with or without glucose. The specific activities of β-glu-curonidase in adherent spleen cells from endotoxin-tolerant ICR mice or genetically resistant C3H/HeJ mice were essentially the same for endotoxin-treated cultures and their untreated counterparts.