The occurrence of nuclear migration under thiol treatment effective in inhibiting neurulation

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Abstract
Early chick embryos were explanted on culture media containing mercaptoethanol and dithiothreitol in concentrations reaching 10−1m and 10−2m respectively. Nuclear migration was found to occur normally even under such thiol concentration, which is more than required to inhibit neurulation. Electron miscroscopy revealed that microtubules were not affected in their number and morphology. However, nuclear migration was blocked with 2·5 × 10−5M colchicine; such treated embryos showed a loss of their microtubules. It is concluded that the two thiols do not act primarily on microtubules when they cause inhibition of neurulation.