Electron Microscopy Study of Chick Embryo Erythrocytes

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Abstract
Micrographs of osmosis-hemolyzed erythrocytes from healthy chick embryos are presented. The cells are examined unfixed, unstained; formalin-fixed, unstained; formalin-fixed, hematoxylin stained; formalin-fixed, methyl green stained; osmic acid-fixed, unstained; and AgNO3-fixed,unstained.'' Unfixed, unstained erythrocytes prepd. by the technic employed showed a transparent cytoplasm so that the direct visualization of intracytoplasmic parasites might be possible by this procedure. Some nuclear structure was observed, and there is evidence for the existence of a cell membrane: fixed erythrocytes clearly showed an envelope. Fixation and drying produced specimen changes, and in the specimens examined the processes of electron microscopy provoked less radical changes than did the fixation.

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