Human immune response to allergens of house dust mite, Dermatophagoides pteronyssinus

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Abstract
From the serum of 10 allergic subjects we have prepared IgG antibodies recognizing idiotopes carried by specific antibodies to Dermatophagoides pteronyssinus (Dpt) allergens, and studied cross‐reactivity of anti‐Dpt IgG bystander and antigen‐binding site‐associated idiotopes by latex agglutination assays. Idiotopes of specific anti‐Dpt IgE were evaluated by radioimmunoassays. Depending on the assay, a binding or inhibition of more than 50%, as compared to the reactivity of specific antibodies with the corresponding anti‐idiotypic (anti‐Id) IgG, was considered significant. Cross‐reactivity of antigen‐binding site‐associated idiotopes attained a mean proportion of 6/10 for IgG and 9.6/10 for IgE. By contrast, bystander idiotopes cross‐reacted only occasionally with a mean proportion of 2/10 for both IgG and IgE antibodies. Anti‐Id antibodies from two subjects have been isolated by adsorption on insolubilized anti‐Dpt antibodies of the corresponding patient. Using this purified material we have confirmed that (a) the majority of anti‐Id antibodies carry an “internal image” of the initial antigen and compete in a dose‐dependent manner with Dpt allergens for the binding to the anti‐Dpt antibodies and (b) paratope‐associated idiotopes of anti‐Dpt antibodies are shared by unrelated individuals.

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