Gas-Liquid Chromatographic Separation of Equine Estrogens

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Abstract
A procedure is described for the separation of equine estrogens by gas-liquid chromatography on a methylphenyl-cyanopropylsilicone column using a combination of trimethylsilyl and methoxyamine-trimethylsilyl derivatization. Resolution of the steroids is achieved on a six-foot column with dual derivatization and dual injection. Partial separation, especially of the difficult to separate estrone and equilin components, can be effected on a twelve-foot column by injection of the trimethylsilyl derivatives alone. The procedure provides a means for identification as well as a possible basis for quantitation of equine estrogens.

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