Quantitative analysis of electron energy‐loss spectra from ultrathin‐sectioned biological material
- 1 April 1991
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 162 (1) , 23-42
- https://doi.org/10.1111/j.1365-2818.1991.tb03113.x
Abstract
A computer program for quantitative spectral analysis is proposed for the elemental analysis of biological material by electron energy-loss spectroscopy in a conventional transmission electron microscope, the Zeiss EM902. Bio-standards are used to test the performance of this program. The application of a simplex optimization method for curve-fitting is proposed to separate the ionization edge from the background. Making use of Ce-, Ca- and Fe-bio-standards, this method is compared with Egerton's well-known two-area method.Keywords
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