Selective and ATP-Dependent Translocation of Peptides by the MHC-Encoded Transporter
- 6 August 1993
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 261 (5122) , 769-771
- https://doi.org/10.1126/science.8342042
Abstract
Major histocompatibility complex (MHC) class I molecules present peptides derived from nuclear and cytosolic proteins to CD8+ T cells. These peptides are translocated into the lumen of the endoplasmic reticulum (ER) to associate with class I molecules. Two MHC-encoded putative transporter proteins, TAP1 and TAP2, are required for efficient assembly of class I molecules and presentation of endogenous peptides. Expression of TAP1 and TAP2 in a mutant cell line resulted in the delivery of an 11-amino acid oligomer model peptide to the ER. Peptide translocation depended on the sequence of the peptide, was adenosine triphosphate (ATP)-dependent, required ATP hydrolysis, and was inhibited in a concentration-dependent manner.Keywords
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