Laser Microfluorometer For Fluorescence Decay Measurements In Biology

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Abstract
A laser microfluorometer is described which is particularly suitable for the measurement of fluorescence decays in microscopic biological samples. The apparatus makes use of (1) a nitrogen pumped dye laser of special design as the excitation source, (2) a microscope system particularly suitable for fluorescence microscopy and (3) an electronic signal averager. The main properties of this instrument include high temporal resolution (~0.2 ns) and high spatial resolution (~0.5μm). This instrument is now being used to study the fluorescence properties of complexes formed by acridine dyes with deoxyribonucleic acid (DNA). Preliminary results indicate that specific information about DNA base-pair sequences and DNA microscopic structure in chromosomes can be obtained.

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