The Membrane Location of Scrapie Infectivity
- 1 June 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 31 (3) , 441-445
- https://doi.org/10.1099/0022-1317-31-3-441
Abstract
Using 5''-nucleotidase and NADPH:cytochrome c reductase as respective enzyme markers for the plasma membrane and endoplasmic reticulum, a satisfactory separation of these 2 membrane fractions from a cell line (SMB) derived from a scrapie mouse brain has been achieved. The coincident distribution of scrapie infectivity and 5''-nucleotidase in various fractions isolated from these cells indicates that most of the scrapie infectivity present in this cell line is associated with the plasma membrane.This publication has 4 references indexed in Scilit:
- Centrifugation Studies on the Infectivities of Cellular Fractions Derived from Mouse Brain Infected with Scrapie ('Suffolk strain')Journal of General Microbiology, 1965
- PLASMA AND CYTOPLASMIC MEMBRANE FRAGMENTS FROM EHRLICH ASCITES CARCINOMAProceedings of the National Academy of Sciences, 1964
- Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphataseBiochemical Journal, 1961
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951