The electrochemical regeneration of NAD+ revisited

Abstract
An actual yield of 99.97% (i.e., 3000 potential regenerating cycles) has been obtained for the electrochemical regeneration of NAD+. The deactivation of the coenzyme is not related to the number of cycles; it is only time dependent. For biotechnological applications, these results show that electrochemical regeneration can compete with enzymatic regeneration for selective oxidation by dehydrogenases.