Does 3'-terminal poly(A) stabilize human fibroblast interferon mRNA in oocytes of Xenopus laevis?
- 1 October 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (10) , 5030-5033
- https://doi.org/10.1073/pnas.75.10.5030
Abstract
Polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase, EC 2.7.7.8) purified from Escherichia coli was used enzymatically to deadenylate polyadenylated human fibroblast interferon mRNA preparations obtained from human diploid fibroblasts (FS-4 strain) induced by poly(I) .cntdot. poly(C) (20 .mu.g/ml) in the presence of cycloheximide (50 .mu.g/ml, 4 h). Both the polyadenylated and the deadenylated interferon mRNA preparations were translated into biologically active human interferon when injected into oocytes to X. laevis. In the oocytes the functional stability to deadenylated interferon mRNA was indistinguishable from that of polyadenylated interferon mRNA.This publication has 23 references indexed in Scilit:
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