CELLULAR AND SUBCELLULAR MECHANISMS OF PHOTODYNAMIC ACTION: THE 1O2 HYPOTHESIS AS A DRIVING FORCE IN RECENT RESEARCH
- 1 October 1978
- journal article
- review article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 28 (4-5) , 493-506
- https://doi.org/10.1111/j.1751-1097.1978.tb06957.x
Abstract
Abstract— Since 1972 the protective (quenching) effect of N3‐ and the enhancing effect of D2O have been used in combination to demonstrate 1O2 (1Δg) as the major reactive intermediate in the dye‐sensitized photooxygenation of biomolecules in in vitro systems. Extended application to in vivo systems has recently begun, producing some results which generally support the involvement of 1O2 pathways in the photosensitization processes. The use of other 1O2 quenchers and acceptors has also been increasing. In the application of these techniques as diagnostic tests for 1O2 participation in in vivo systems, careful examination of the experimental conditions should be made with respect to the quenching capability and accessibility of D2O in the specified cellular environment. Furthermore, the diffusive nature of 1O2, generation sites, generation efficiencies, the location and type of sensitizers, and the reactivity of 1O2 with presumed target structures in the cell should also be taken into account in the interpretation. Recent studies illustrating the importance of these factors are discussed. Finally, a tentative picture of the mode of in vivo photodynamic activity of common dye sensitizers is outlined.This publication has 111 references indexed in Scilit:
- Reactivity of singlet molecular oxygen with cholesterol in a phospholipid membrane matrix. A model for oxidative damage of membranesBiochemical and Biophysical Research Communications, 1977
- Induction of base-pair substitution and prameshift mutations in wild-type and repair-deficient strains of Salmonella typhimurium by the photodynamic action of methylene blueMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- Action of light on Micrococcus roseusCanadian Journal of Microbiology, 1974
- Induction of frameshifts and base-pair substitutions by acridine orange plus visible light in bacteriaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Some differences between 470 nm and 510 nm in the acridine-orange-sensitized photodynamic actions on yeast cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Über die RSN-Synthese bei Saccharomyces-Zellen nach photodynamischer Behandlung und nach RöntgenbestrahlungRadiation and Environmental Biophysics, 1973
- Ultraviolet-killing and photodynamic-killing in mutants of Escherichia coliMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- Dark repair of acridine dye-sensitized photoeffects in E.coli cells and bacteriophageBiochemical and Biophysical Research Communications, 1968
- Die Wirkung von Thiopyronin auf die Dunkelreaktivierung von UV-, Röntgen- und photodynamischen Schäden beiSaccharomycesRadiation and Environmental Biophysics, 1968